Coding

Part:BBa_K4716996

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


Fvp-p (Flammulina velutipes prolyl endopeptidase)

Fvp is a prolyl endopeptidase gluten breaking enzyme from Enokitake (Flammulina velutipes), which can break down the 33-mer toxic peptide of gluten for celiac disease patients that can not be completely broken down in the intestine more efficiently. We sequence it to be expressed in E. coli and secrete the enzyme outside the gut microbiota.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10
    Illegal BsaI site found at 355
    Illegal BsaI site found at 622
    Illegal SapI site found at 511


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Categories
Parameters
None